Phylogenic identification of toxigenic Bacillus cereus in chili and white pepper from Bogor area, Indonesia

This study aimed to compare the occurrence and level of Bacillus cereus sensu stricto (B. cereus) in chili, previously isolated white pepper from traditional supermarkets around Bogor, determine phylogenetic relationship between obtained isolates based on their 16S rDNA gene, potential toxicity ces nheA genes using Polymerase Chain Reaction. The highest presumptive B. samples was 5.95×105 2.6×105 CFU/g respectively. difference levels two market types not significant. Ten chili 10 our previous were sequenced, subjected BLAST analysis, 13 confirmed as lato. sequences phylogenetically analysed tested for possession toxigenic genes. Based tree established, 12 related cereus, sharing >98% similarity with reference strains. All (100%) owned gene; none them possessed gene. Absence gene lessens danger emesis these spices, nonetheless, 100% presence presents a risk diarrheal syndrome.